mellowtigger: (pikachu magnifying glass)
mellowtigger ([personal profile] mellowtigger) wrote 2022-04-12 09:13 pm (UTC)

P.S. This detailed rebuttal from your middle link is hopefully a little good news?

"The very low frequency of identified chimeric events (Table 1) suggests that SARS-CoV-2 integration into the host genome is unlikely. Given that the HEK293T-L1 model increases detection of “rare integration events” and L1 can retrotranspose any polyadenylated cellular RNA (2) and “insertions” are found preferentially in protein-coding exons, a bias unknown to L1 endonuclease insertions (3), these findings are likely spurious. Additionally, 2 of the identified 61 chimeric nanopore genomic DNA (gDNA) reads contain human DNA from separate chromosomes (chr1,chr22 and chr18,chrX, respectively), suggesting a portion of chimeric gDNA nanopore reads have arisen due to infrequent technical artifacts, such as base-calling software not recognizing an open-pore state between distinct molecules."

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